This course aims to enable students :

1.1 Describe and understand the structure, function and biochemical importance of macro-, micronutrients, hormones and enzymes.

1.2 describe and relate the metabolic pathways of macronutrients and nucleotides.

1.3 illustrate the contribution of the organs in metabolic process under different physiological circumstances.

1.4 Identify and use  biotechnology methods and tools  and their clinical implication in the field of Medical Biochemistry and Molecular Biology.

1.5 Describe structure and function of the subcellular structures e.g. nucleus, mitochondria.

1.6  Correlate  hereditary and acquired metabolic disturbances with biochemical and molecular basis and understand their biochemical laboratory and clinical implications.

1.7 Apply analytical method and criticism of knowledge in medical biochemistry and related fields.

1.8 Interpret medical laboratory reports.

1.9 Practice   practical, analytical skills necessary for proper detection of ongoing provisional problems and find innovative solutions for them.

1.10 Act with integrity and credibility and follow the ethical code of medical practice.

1.11  Participate in community progress & assess and solve  environmental health problems.

1.12 Master wide range of skills and be aware of  safe laboratory practice.

1.13 Take decision based according to availability of data .   

1.14 Communicate with others and lead them properly with team spirit.

1.15 Continues education with addition to knowledge and transfer of this knowledge and experience  to the others.

2- الهدف من المقرر

1-    المستهدف من تدريس المقرر:

By the end of the course the students should be able to:

1. Describe the metabolic pathways of the main dietary sources of energy: carbohydrates, fats and proteins, their digestion absorption, their oxidation to release energy.
2. Illustrate the regulation of these pathways and the integration of their metabolism.
3. Identify biochemical alteration in related metabolic disorders.
4. Illustrate the biochemistry of certain tissues like liver, kidney, muscles, cartilage, bone and nervous system and explain the role of vitamins and enzymes required for catalysis of these processes, in addition to the deficiency manifestation of each.
5. Describe the metabolism of the major minerals and trace elements their functions and alterations in metabolic processes met with in the deficiency or excess of these elements
6. Describe the components of some body fluids e.g. blood, urine, milk, semen, CSF and sweat
7. Acquire knowledge about nucleic acid metabolism with special  emphasis on their role in protein synthesis
8. Describe the structure of  DNA and RNA as well as the processes of replication, transcription, translation,
9. discuss principles and techniques molecular biology and their medical applications
10.  List the characteristics of the genetic code.
11. Identify various types of mutations and their relation to genetic diseases and cancers.
12. Describe structure and functions of biological membranes and their role in transport  and in biochemical , clinical and laboratory importance.
13. Recall effect of his clinical practice on environment and principles of environmental development and saving.
14. identify  most recent advances, in selected areas relevant to each subject.
15. explain scientific background  and function of  laboratory equipment and methods used in Medical Biochemistry and Molecular Biology.
16. discuss different techniques and tools for searching the scientific literature.
17. identify the basics of ethics and scientific, medico legal aspects of health problems and clinical practice with special emphasis on biomedical investigations.

أ- المعلومات والمفاهيم :

By the end of the course the students should be able to :

1. Integrate various metabolic pathways together with their regulation.
2. Correlate metabolic role of certain organs (liver, kidney ,brain and CNS) to function in health and disease.
3. Search for primary and secondary scientific literature relevant to a specific topic.
4. Analyze and interpret the results of various medical investigations related to Medical Biochemistry.
5. Suggest accurately possible biochemical and /or molecular investigations needed for diagnosis.
6. Solve problems related to Medical Biochemistry and Molecular Biology in a given case study report.
7.  Formulate hypotheses and design experiments to test these hypotheses and carry out research studies.
8. Take provisional decision based on knowledge in the field of Medical Biochemistry.
9. Plan for continuous development of performance in the field of Medical Biochemistry and Molecular Biology.
10. Evaluate of hazards in clinical practice, safety procedures.

ب- المهارات الذهنية  :

1. Perform various investigations and techniques related to Medical Biochemistry efficiently.
2. Use competently relevant laboratory equipment.
3. Apply and Interpret results of basic techniques used in molecular biological tests.
4. Master numerical, graphical, statistical and other methods for analyzing experimental data.
5. Write up and interpret reports related to Medical Biochemistry  investigations.
6. Formulate and write scientific papers in the area of Medical Biochemistry and Molecular Biology.
7. Apply safety measure in the laboratory.
8. Use of technological methods to serve the professional practice.
9. Plan for development of practice . 

ج- المهارات  المهنية :

1. Communicate properly with the staff members as well as with each other.
2. Respect the rules of laboratory work.
3. Work effectively as member or leader to team.
4.   Manage his own learning, including time management skills and learn effectively from a range of resources. 
5. Use different teaching and evaluation methods to teach others and give feedback on their performance.
6. Perform scientific meetings according to the available time.
7.  Use  various technological methods and tools to gain information.
8. Make use of available resources to learn independently and continuously.
9. Discuss and dialogue based on reasoning and evidence.
10. Retrieve and compare properly the biochemical information from a variety of sources.

د- المهارات العامة:

محتوى المقرر

Subjects

lectures

practical

Total

% of total

DNA: The replicative process & repair

60

---

4.76

RNA: Structure, transcription and posttranscriptional modification

60

---

4.76

Protein synthesis: translation and posttranslational modifications

120

---

9.5

Recombinant DNA and Biotechnology

60

---

4.76

Regulation of gene expression

60

---

4.76

Eucaryotic Cell structure

60

---

4.76

Proteins: composition & structure, Structure-function relationship of protein families

60

---

4.76

Enzymes: classification, kinetics, control

60

---

4.76

Carbohydrate metabolism: Major and special pathways, and their control.

120

---

9.5

Lipids: Utilization, storage metabolism of special lipids

120

---

9.5

Amino Acids: General pathways and individual a.a. metabolism

120

---

9.5

Metabolic interrelations

60

---

4.76

Biochemistry of peptide and steroid hormones 

60

---

4.76

Molecular cell biology, biotransformations, the cytochrome P-450

60

---

4.76

Iron and Haem Metabolism,Gas Transport and PH Regulation

60

---

4.76

   Digestion and Absorption of basic nutritional constituents

60

---

4.76

Principles of  nutrition, macronutrients and micronutrients 

60

---

4.76

Subcellular fractionation- Nutrition- and 3 out of the following six topics: Biochemistry of vision, of connective tissue, of liver, of muscle of nervous tissue, and of adipose tissue.

240

---

16

Spectrophotometry- Flame photometry- chromatography- Electrophoresis- Colorimetric assays- Competitive binding assays (RIA, ELISA molecular Biology techniques

240

----

100

1. lectures.

2. Problem solving.

3. Self-learning.

4. Practical & clinical classes.

5- اساليب التعلم والتعليم

6- تقويم الطلاب

مخرجات التعلم المستهدفة

الوسيلة

أ-الاساليب المستخدمة

To assess knowledge & intellectual skills.

To assess knowledge, intellectual skills & General & transferable skills

To assess Practical & Clinical skills

Written examination

Oral examination

Practical examination

1

2

3

Two written exam+ oral exam + practical exam at the end of the course

ب- التوقيت

1- Written exam

a) First paper

b) Second paper

2- oral exam

3 -practical exam

350

350

200

100

ج‌-    توزيع الدرجات

قائمة الكتب الدراسية والمراجع

Harper’s Biochemistry by:  Murray, R K, Bender, DA., Botham, KM., Kennelly, PG., Rondwell, VW., Weil, PA : Harper's Illustrated Biochemistry (28th edition), McGraw Hill companies, Inc. (2009).

أ‌-كتب ملزمة

Lippincott’s illustrated Biochemistry by Champe, PC., Harvey, RA., Ferrier, DR : Lippincott's Illustrated Reviews: Biochemistry (4th edition), Lippincott 's Williams &Wilkins (2008).

ب - كتب مقترحة

Periodical websites : www.clinchem.org.

ج- دوريات علمية